Isolation and Characterization of Long-term Embryogenic Lines in Asparagus officinalis L.
Identifieur interne : 000248 ( France/Analysis ); précédent : 000247; suivant : 000249Isolation and Characterization of Long-term Embryogenic Lines in Asparagus officinalis L.
Auteurs : B. Delbreil [France] ; I. Goebel-Tourand [France] ; C. Lefrançois [France] ; M. Jullien [France]Source :
- Journal of Plant Physiology [ 0176-1617 ] ; 1994.
English descriptors
Abstract
Summary: Somatic embryogenesis was obtained from 14 Asparagus officinalis L. genotypes by in vitro culture of different explant types such as cladophylls, apices and isolated mesophyll cells. Explants first induced on a culture medium containing growth regulators produced calli and developed few somatic embryos when transferred on a hormone-free medium. During this development phase, we isolated a new type of friable tissues that produced abundant somatic embryos. The frequencies of emergence of these highly embryogenic tissues were estimated between 10−5 and 10−6. These tissue were habituated; being established as long-term embryogenic lines by repeated subcultures on hormone-free medium, continuously they produced numerous somatic embryos, some of them converting to whole plants. Histological data showed these long-term embryogenic lines to grow by recurrent embryogenesis arising, from single epidermal cells of preexisting embryos.
Url:
DOI: 10.1016/S0176-1617(11)80543-2
Affiliations:
- France
- Centre-Val de Loire, Région Centre, Île-de-France
- Paris, Soings en Sologne, Versailles
- Université Pierre-et-Marie-Curie
Links toward previous steps (curation, corpus...)
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Links to Exploration step
ISTEX:14A950CCFA9CF06C1FF05A52BE9D1668C64C4275Le document en format XML
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